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KMID : 0900919980220010073
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Korean journal of Animal Reproduction
1998 Volume.22 No. 1 p.73 ~ p.80
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Interaction between Transforming Growth Factor beta and Cumulus Cells during In Vitro Maturation in Porcine Oocytes
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Shin Myung-Kyun
Cho Je-Won
Cheong Hee-Tae
Yang Boo-Keun
Kim Choung-Ik
Park Choon-Keun
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Abstract
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This study was undertaken to evaluate the interaction between cumulus cells and TGF beta1 on in vitro maturation in porcine oocytes. No differ ences were found in maturation rates when follicular oocytes were cultured in medium with various concentrations of TGF beta. At 24 h after maturation, the oocytes matured to metaphase-II were found in medium with TGF beta regardless of cumulus cells. On the other hand, the maturation rates were significantly(P < 0.01 higher cumulus-enclosed(70 and 52%) than cumulus-denuded oocytes(35 and 26%) in medium with or without TGF beta at 48 h after culture. In a another experiment, the same maturation rates (54-71%) were observed when cumulus-enclosed oocytes were cultured with various addition time of TGF beta. However, the maturation rates in cumulus-denuded oocytes were significantly (P < 0.05) higher in medium added at 0~24 h (59%) or 24-48 h(57%) after culture than in medium with(27%) and without(38%) TGF beta for 48 h. These results indicated that cumulus cells is essential for in vitro maturation in porcine oocytes but TGF beta can promote oocytes maturation in cumulus-free oocytes.
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KEYWORD
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Porcine oocytes, In vitro, Maturation, TGF beta, cumulus cells
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